2024

Fluorescence Microscopy and Immunoblotting for Mitophagy in Budding Yeast
Yuki Nakayama, Koji Okamoto

Methods Mol Biol. 
2024:2845:1-14. （PMID: 39115653 DOI: 10.1007/978-1-0716-4067-8_1）

2024

The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast 
 Yuan Tian, Koji Okamoto

Sci Rep.
2024 Apr 11;14(1):8477.（PMID: 38177147 DOI: 10.1038/s41598-023-50245-7）

2023

A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis 
Toan Khanh Le, Yasuhiro Hirano, Haruhiko Asakawa, Koji Okamoto, Tatsuo Fukagawa, Tokuko Haraguchi, Yasushi Hiraoka

J Cell Sci
 (2023) 136 (19): jcs260930. （PMID: 37694715 DOI: 10.1242/jcs.260930）

2023

The GET pathway serves to activate Atg32-mediated mitophagy by ER targeting of the Ppg1-Far complex 
Mashun Onishi, Mitsutaka Kubota, Lan Duan, Yuan Tian, Koji Okamoto

Life Sci Alliance
 2023 Jan 25;6(4):e202201640. （PMID: 36697253 DOI: 10.26508/lsa.202201640）

2022

An overview of the molecular mechanisms of mitophagy in yeast .  Schuster R, Okamoto K

Biochimica et Biophysica Acta - General Subjects 2022 Nov;1866(11):130203. （PMID: 35842014 DOI: 10.1016/j.bbagen.2022.130203）

2021

The protein N-terminal acetyltransferase A complex contributes to yeast mitophagy via promoting expression and phosphorylation of Atg32.  Mitsutaka Kubota, Koji Okamoto

J. Biochem. 170(2):175-182  2021 （PMID：34115119 DOI：10.1093/jb/mvab068）

Mitochondrial dynamics and degradation in the oleaginous yeast Lipomyces starkeyi. Lan Duan, Koji Okamoto

Genes Cells 26(8):627-635  2021 （PMID：34085353 DOI：10.1111/gtc.12875）

2020

Detection of mitophagy in mammalian cells, mice, and yeast. Calvelli Hannah, Krigman Judith, Onishi Mashun, Narendra Derek P, Sun Nuo, Okamoto KojiMethods in Cell Biology 155 557 - 579 2020年

マイトファジーの分子機構と生理的意義 (第5土曜特集 オートファジー : 分子機構・生物学的意義・疾患との関わり) -- (オートファジーの生物学的意義)
大西 真駿, 岡本 浩二医学のあゆみ 272(9) 801 - 810 2020年2月29日

2019

Repression of mitochondrial metabolism for cytosolic pyruvate-derived chemical production in Saccharomyces cerevisiae. Morita Keisuke, Matsuda Fumio, Okamoto Koji, Ishii Jun, Kondo Akihiko, Shimizu HiroshiMicrobial Cell Factories 18(1) 177 - 177 2019年10月15日 

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy. Zheng Liangde, Shu Wen-Jie, Li Yu-Min, Mari Muriel, Yan Chaojun, Wang Dehe, Yin Zhao-Hong, Jiang Wei, Zhou Yu, Okamoto Koji, Reggiori Fulvio, Klionsky Daniel J, Song Zhiyin, Du Hai-NingAutophagy 1 - 14 2019年9月19日 

A Mammalian Mitophagy Receptor, Bcl2-L-13, Recruits the ULK1 Complex to Induce Mitophagy. Murakawa Tomokazu, Okamoto Koji, Omiya Shigemiki, Taneike Manabu, Yamaguchi Osamu, Otsu KinyaCell Reports 26(2) 338 - 345 2019年1月  

2018

Mitochondrial stasis reveals p62-mediated ubiquitination in Parkin-independent mitophagy.

Yamada T, Murata D, Adachi Y, Itoh K, Kameoka S, Igarashi A, Dawson TM, Yanagawa T, Okamoto K, Iijima M, *Sesaki H.

Cell Metab., 28(4):588-57 (2018).

The TORC1 signaling pathway regulates respiration-induced mitophagy in yeast.

Liu Y, *Okamoto K.

Biochem. Biophys Res Commun. , doi: 10.1016/j.bbrc.2018.04.114(2018).

The ER membrane insertase Get1/2 is required for efficient mitophagy in yeast.

Onishi M, Nagumo S, Iwashita S, *Okamoto K.

Biochem. Biophys Res Commun., doi: 10.1016/j.bbrc.2018.05.123 (2018).

The Nem1-Spo7 protein phosphatase complex is required for efficient mitophagy in yeast.

Xu X, *Okamoto K.

Biochem. Biophys Res Commun., 496(1):51-57 (2018).

<Reviews, Proceedings and Books>

Phosphatidic acid and cardiolipin coordinate mitochondrial dynamics.

Kameoka S, Adachi Y, Okamoto K, Iijima M, *Sesaki H.

Trends Cell Biol., 28: 67-76. (2018).

分解標識タンパク質が駆動するミトコンドリア特異的オートファジー

*岡本浩二

DOJIN NEWS, No. 165, 7-9., ISSN 0385-1516 (2018).

2017

Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting.

Nagumo S, *Okamoto K.

Methods Mol Biol., doi: 10.1007/7651_2017_11 (2017).

Assays for Mitophagy in Yeast.

Eiyama A, *Okamoto K.

Methods Mol Biol., 1567:337-347. (2017)

<Reviews, Proceedings and Books>

ミトコンドリア分別・除去システムの基本原理

*岡本浩二

The Frontiers in Life Sciencesシリーズ、「オートファジー」, 113-123., ISBN 978-4-525-13481-5 (2017).

2015

Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling

Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, *Okamoto K.

EMBO J. 34: 2703-2719. (2015)

Protein N-terminal acetylation by the NatA complex is critical for selective mitochondrial degradation.

Eiyama A, *Okamoto K.

J. Biol. Chem. 290: 25034-25044.(2015)

<Reviews, Proceedings and Books>

Preface - The special issue on mitophagy.

*Okamoto K.

Biochim. Biophys. Acta 1853: 2755. (2015)

PINK1/Parkin-mediated mitophagy in mammalian cells.

Eiyama A, *Okamoto K.

Curr. Opin. Cell Biol. 33: 95-101.(2015)

2014

Enhancement of ethanol fermentation in Saccharomyces cerevisiae sake yeast by disrupting mitophagy function.

Shiroma S, Jayakody LN, Horie K, Okamoto K, *Kitagaki H.

Appl. Environ. Microbiol.80: 1002-1012.(2014)

<Reviews, Proceedings and Books>

Receptor-mediated mitophagy in yeast and mammalian systems.

Liu L, Sakakibara K, *Chen Q, *Okamoto K.

Cell Res. 24: 787-795.(2014)

Organellophagy: eliminating cellular building blocks via selective autophagy.

*Okamoto K.

J. Cell Biol. 205: 435-445.(2014)

Assays for autophagy II: Mitochondrial autophagy.

*Kanki T, Okamoto K.

Methods Mol. Biol. 1163: 165-173.(2014)

出芽酵母が語る選択的ミトコンドリア分解の基本原理.

*岡本浩二.

医学のあゆみ・AYUMI「マイトファジー―基礎から疾患との関連まで」 250: 483-487.(2014)

2013

Mitochondrial degradation during starvation is selective and temporally distinct from bulk autophagy in yeast.

Eiyama A, Kondo-Okamoto N, *Okamoto K.

FEBS Lett. 587: 1787-1792.(2013)

<Reviews, Proceedings and Books>

No ATG is an island - the connection of autophagy with diverse pathways and functions.

*Komatsu M, Kuma A, Okamoto K.

EMBO Rep. 14: 219-221.(2013)

2012

Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy.

Kondo-Okamoto N, Noda NN, Suzuki SW, Nakatogawa H, Takahashi I, Matsunami M, Hashimoto A, Inagaki F, Ohsumi Y, *Okamoto K .

J. Biol. Chem.287: 10631-10638.(2012)

<Reviews, Proceedings and Books>

Mitochondria and autophagy: critical interplay between the two hemostats.

Kondo-Okamoto N, *Okamoto K.

Biochim. Biophys. Acta 1820: 595-600.(2012)

マイトファジーの分子機構.

*岡本浩二

DOJIN BIOSCIENCE SERIES 04，「オートファジー」. 133-149.(2012)

ミトコンドリア品質管理研究の最前線.

*岡本浩二

細胞 THE CELL 44: 45-48．(2012)

2011

Structure-function analysis of the yeast mitochondrial Rho GTPase, Gem1p: implications for mitochondrial inheritance.

Koshiba, T., Holman, H.A., Kubara, K., Yasukawa, K., Kawabata, S., Okamoto, K., MacFarlane, J., Shaw, J.M.

J. Biol. Chem. 286: 354-362. (2011)

<Reviews, Proceedings and Books>

Mitochondria breathe for autophagy.

Okamoto, K.

EMBO J. 30: 2095-2096.(2011)

Mitochondria autophagy in yeast.

Kanki, T., Klionsky, D.J., Okamoto, K.

Antioxid. Redox Signal.14:1989-2001.(2011)

2010

<Reviews, Proceedings and Books>

酵母のマイトファジー〜観察からの始まり〜．顕微鏡

岡本徳子，岡本浩二，大隅良典

45: 83-86．(2010)

マイトファジー：ミトコンドリアを丸ごと分別・除去する仕組み．細胞工学・特集「その時ミトコンドリアは動いた」

岡本浩二*，岡本徳子

細胞工学・特集「その時ミトコンドリアは動いた」29: 423-428．(2010)

2009

Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy.

Okamoto, K.*, Kondo-Okamoto, N., and Ohsumi, Y.*

Dev. Cell,17: 87–97.(2009)

<Reviews, Proceedings and Books>

A landmark protein essential for mitophagy. Autophagy,

Okamoto, K.*, Kondo-Okamoto, N., and Ohsumi, Y.*

Autophagy, 5: 1203-1205.(2009)

2008

TPR proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis.

Kondo-Okamoto, N., Shaw, J.M., and Okamoto, K.*

EMBO rep., 9: 63-69.(2008)

Multiple pathways influence mitochondrial inheritance in budding yeast.

Frederick, R.L., Okamoto, K., and Shaw, J.M.

Genetics, 178: 825-837.(2008)